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    The first International ReGenera R&D International for Aging Intervention and VCC Preventive Medical Promotion Foundation has been a flashy success.

    It has been held in Beijing last June 8, having as topics Gut Microbiota and Health. The keynote speaker was prof. Marotta and the meeting features the most authoritative Asian specialists in the field.

    The conference room was fully packed and, most important, about 10.000 physicians and health professionals registered for a live attendance at distance.

    The event as covered by the most reputed national TV channels and in the next days a series of interviews followed up.

    The organizer, VCC Preventive Medical Promotion Foundation, given this success, is planning further similar top scientific gatherings this year in other locations.

    There was a general consensus on the importance to deepen our knowledge in this area as well ass starting providing practical steps forwards an evaluation and phenotypic monitoring of gut microbiota, while envisaging designer pro-prebiotics interventions.

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Old age and renal susceptibility to drugs. High-purity green tea ameliorates renal oxidative damage induced by gentamicin in aged rats

February 7, 2011

F Marotta1, Y Naito2, A. Lorenzetti1, A Kumari3, P Signorelli1, C Tomella1, A Polimeni1,
1ReGenera. Research Group for Aging-Intervention, Milano, Italy; 2Seyukai Medical Institute & Clinic, Nagoya, Japan; 3Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey.

Up to 30% of patients treated with gentamicin (GM) for more than seven days show some signs of nephrotoxicity and this holds particularly relevant in elderly people. It has been reported that GM-induced nephrotoxicity is characterized by direct tubular necrosis, which is localized mainly in the proximal tubule. The exact mechanism of GM-induced nephrotoxicity is unknown. However, GM has been shown to enhance the generation of ROS causing increased consumption of GSH in non-enzymatic removal of oxygen-radicals. Therefore, GM may act like S-(1,2-dichlorovinyl)-L-cysteine a known nephrotoxicant and interferes with the recycling of GSSG into GSH by inhibition of the enzyme glutathione reductase. Green tea has reported to have antioxidant and free radical scavenging activities through its important catechins contents. Therefore, the aim of this work was to investigate the possible protective effect of a high-purity green extract (GTE)(Mega Green Tea Extract® containing decaffeinated leaf with 98% polyphenols and 45% epigallocathechin-gallate, LEF Ft Lauderdale, USA) against gentamicin-induced nephrotoxicity. For this purpose, 18-month old rats were divided into 4 groups. Group-1 (control) received normal saline. Group-2 received GTE (300 mg/kg/d, orally). Group-3 received gentamicin (80 mg/kg/d, intraperitoneally). Group-4 was injected with GTE plus gentamicin simultaneously. Daily urinary total protein levels were estimated to assess kidney dysfunction. The rats were sacrificed on the seventh day and kidneys were collected for histopathological studies. Blood urea nitrogen (BUN) and creatinine levels were measured in the blood. Moreover, GSH, TBARS levels, SOD and catalase activities were determined in renal tissues. GM produced elevation in urinary total protein, BUN, serum creatinine and TBARS levels. On the other hand, GM reduced the GSH level and SOD, CAT activities. GM-treated rats showed atrophy/hypertrophy of the glomeruli with degeneration and necrosis in the epithelial cells lining of renal tubules. The endothelial cells lining the glomerular tufts showed swelling and there was cytoplasmic vacuolation and focal inflammatory cells infiltration between the tubules in the corticomedullary junction and in the perivascular area of the dilated blood vessels associated with oedema. GTE reversed most of the histopathological alterations induced by GM and was found to be safe in healthy kidney. Accordingly, the administration of GTE plus GM significantly protected kidney function with normalization of all biochemical parameters (p<0.01).

Our results were consistent with the suggestion that high-quality GTE may indeed represent an effective protective agent from GM-induced kidney damage by exerting significant antioxidant and membrane lipid protective properties from oxidative/inflammatory injury.
Cuzzocrea S, Mazzon E, Dugo L, Serraino I, Di Paola R, Britti D, De Sarro A, Pierpaoli S, Caputi A, Masini E and Salvemini D (2002). A role for superoxide in gentamicin-mediated nephropathy in rats. Eur. J. Pharmacol., 450: 67-76.

Maldonado PD, Barrera D, Medina-Campos ON, Hernández-Pando R, Ibarra-Rubio ME and Pedraza- Chaverrí J (2003). Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats. Life Sci., 73: 2543-2556.

Yanagida C, Ito K, Komiya I and Horie T (2004). Protective effect of fosfomycin on gentamicin-induced lipid peroxidation of rat renal tissue. Chem. Biol. Interact., 148: 139-147.

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    The first International ReGenera R&D International for Aging Intervention and VCC Preventive Medical Promotion Foundation has been a flashy success.

    It has been held in Beijing last June 8, having as topics Gut Microbiota and Health. The keynote speaker was prof. Marotta and the meeting features the most authoritative Asian specialists in the field.

    The conference room was fully packed and, most important, about 10.000 physicians and health professionals registered for a live attendance at distance.

    The event as covered by the most reputed national TV channels and in the next days a series of interviews followed up.

    The organizer, VCC Preventive Medical Promotion Foundation, given this success, is planning further similar top scientific gatherings this year in other locations.

    There was a general consensus on the importance to deepen our knowledge in this area as well ass starting providing practical steps forwards an evaluation and phenotypic monitoring of gut microbiota, while envisaging designer pro-prebiotics interventions.

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